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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial role in promoting cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two added splice variants ended up reported but in depth nucleotide sequence information is just not nevertheless readily available.
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Blocking peptides are peptides that bind especially towards the concentrate on antibody and block antibody binding. These peptide usually contains the epitope recognized from the antibody.
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This information outlines even further doable optimization of cellular and immunoassay parameters to make certain the best possible results are...
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This guidebook outlines further more achievable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
Antibodies sure to the blocking peptide now not bind to your epitope on the goal protein. By evaluating the staining in the blocked antibody vs . the antibody alone, one can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs into the highly conserved cyclin household, whose customers are characterized by a spectacular periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which contribute to your temporal coordination of each and every mitotic occasion. Cyclin E types a complex with and features for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is also degraded as cells development by S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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